embryonic stem cell line h9  (WiCell Research Institute Inc)

Journal: bioRxiv

Article Title: Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit

doi: 10.64898/2026.04.23.720508

Figure Lengend Snippet: (a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A H9 hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.

Article Snippet: The human embryonic stem cell line H9 (WA09, WiCell) and human iPSCs lines GM25256 (Coriell Institute) and KOLF2.1J (The Jackson Laboratory) were used to generate the base model for this study.

Techniques: Membrane

Journal: Advanced Science

Article Title: Comprehensive Profiling of N 6 ‐methyladnosine (m 6 A) Readouts Reveals Novel m 6 A Readers That Regulate Human Embryonic Stem Cell Differentiation

doi: 10.1002/advs.202510075

Figure Lengend Snippet: Assessment of the regulatory effects of m 6 A‐binding proteins and m 6 A readers on hESC proliferation and differentiation. (A) Representative images of morphology of WT versus FUBP3 , FXR2 , and L1TD1 KO hESCs under bright field. (B) Growth curve assessing cell proliferation kinetics of FUBP3 , FXR2 , and L1TD1 KO versus WT hESCs. (C) ALP staining assay of WT versus FUBP3 , FXR2 , and L1TD1 KO hESCs. (D) Schematic representation of the induction endodermal differentiation experiment of hESCs. (E‐F) Flow cytometry and statistical histogram analysis of endodermal differentiation of WT versus FUBP3 , FXR2 and L1TD1 KO hESCs. (G) Schematic representation of the induction hematopoietic mesodermal differentiation experiment of hESCs. (H‐I) Flow cytometry and statistical histogram analysis of hematopoietic mesodermal differentiation of WT versus FUBP3 , FXR2 and L1TD1 KO hESCs. (J) Schematic representation of the induction neural ectodermal differentiation experiment of hESCs. (K‐L) Flow cytometry and statistical histogram analysis of neural ectodermal differentiation of WT versus FUBP3 , FXR2 and L1TD1 KO hESCs. (M‐O) RT‐qPCR analysis of definitive endoderm markers SOX17, FOXA2 (M); mesoderm marker TBXT (also known as T) and CD34 (N); and neural‐ectoderm markers SOX1, PAX6, NES in WT versus FUBP3 , FXR2 and L1TD1 KO hESCs (O). Figures in (D), (G), and (J) were drawn with Biovisart ( https://biovisart.com.cn ) and Bioicons ( https://bioicons.com/ ) with permission. Data in (B), (F), (I), (L), and (M‐O) were statistically analyzed using two‐tailed unpaired Student's t ‐test. Error bars represent mean ± SD ( n = 3 independent experiments). For all statistical plots, * means p < 0.05, ** means p < 0.01, *** means p < 0.001, **** means p < 0.0001, ns means not significant.

Article Snippet: The human embryonic stem cell (hESC) line H9 (WA09) was obtained from WiCell Research Institute (USA, catalog#WA09, RRID: CVCL_9773) in 2019 under appropriate material transfer agreements.

Techniques: Binding Assay, Staining, Flow Cytometry, Quantitative RT-PCR, Marker, Two Tailed Test